The maximum stability for the five 3' bases of a left or right primer. The value is the maximum delta G for duplex disruption for the five 3' bases as calculated using the nearest neighbor parameters published in Breslauer, Frank, Bloeker and Marky, Proc. GSP will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt. Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo. The minimum, optimum, and maximum melting temperature of the amplicon.
GSP will not pick oligos with temperatures smaller than Min or larger than Max, and with default conditions will try to pick primers with melting temperatures close to Opt. GSP will not pick a product with melting temperature less than min or greater than max.
The 3'-anchored global alignment score is taken to predict the likelihood of PCR-priming primer-dimers, for example The scoring system is as for the Max Complementarity argument.
In the examples above the scores are 7.00 and 6.00 respectively.
The value for this parameter has the form This parameter should be considered EXPERIMENTAL at this point.